Asian Journal of Research in Chemistry

N. I. Kochar ¹, M. N. Dahake ¹, R. L. Bakal ¹, A. P. Devani ¹, A. V. Chandewar ¹

Affiliations
1 Department of Quality Assurance, P.W. College of Pharmacy, Dhamangaon Road, Yavatmal, 445001, IN

Abstract

A simple isocratic RP-HPLC method has been developed and subsequently validated for the determination of Ramipril and Amlodipine Besylate in pharmaceutical dosage forms within very short retention time. The method employs an Xterra C18 column, 5 μ, 150 mm x 4.60 mm id with flow rate of 1.5 ml/min using UV detection at 210nm. The separation was carried out using a mobile phase consisting of Sodium Lauryl Sulfate buffer by adjusting pH 2.5 and final composition is Buffer: Acetonitrile: Methanol (45:16.5:38.5)V/V. The retention time for Ramipril and Amlodipine Besylate was found to be 3.1 minutes and 3.8 minutes respectively. The results of analysis were validated statically and by recovery studies. Hence the proposed method was found to be accurate, precise, reproducible and specific and can be used for simultaneous analysis of these drugs in tablet formulation.

Keywords

RP-HPLC, Sodium Lauryl Sulfate Buffer, Ramipril, Amlodipine Besylate.

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R. S. Sakhare ¹, A. B. Roge ¹, R. L. Bakal ¹, A. D. Dewani ¹, M. D. Kshirsagar ¹, A. V. Chandewar ¹

Affiliations
1 P. Wadhwani College of Pharmacy, Yavatmal-445001 (M.S.), IN

Abstract

A simple, precise, accurate, rapid and reproducible reverse phase high performance liquid chromatographic procedure was developed for simultaneous determination of Drotaverine HCL and Mefenamic Acid in tablet dosage form at a single wavelength. The mobile phase used was a combination of Methanol:Acetonitrile:KH₂PO₄ Buffer (10 mM):(40:40:20) pH 3.5. The detection of the combined dosage form was carried out at 240 nm and flow rate was set to 1ml/min. Linearity was obtained in the concentration range of 3 to 30 μg/ml of Drotaverine HCL and 6 to 33 μg/ml of Mefenamic Acid with correlation coefficients of 0.9998 and 0.9999, respectively. The results of the analysis were validated statistically and recovery studies confirmed the accuracy of the proposed method.

Keywords

Drotaverine HCL, Mefenamic Acid, RP-HPLC, Simultaneous Estimation.

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Rakhee K. Kotecha ¹, Anand S. Surana ², A. V. Chandewar ¹, P. D. Nakhat ³, N. P. Jogad ⁴

Affiliations
1 Department of Pharmaceutics, P.W. College of Pharmacy, Yavatmal, Maharashtra, IN
2 Department of Pharmacology, S.K.B College of Pharmacy, Kamptee, Maharashtra, IN
3 Pharma Research, Wochardt Research Centre, Aurangabad, IN
4 Formulation and Development, Inventia Health Care Pvt. Ltd., Thane, IN

Abstract

The main aim of the present study was to developed analytical method for phenylpropanolamine hydrochloride (PPH) in PPH sustained release pellets. Analysis of PPH i.e. assays and dissolution has been given in USP by HPLC method. No UV-Visible spectrometry method has been reported for the analysis of PPH. Analysis of PPH was carried out by using UV-Visible spectrometry after performing the part method validation of specificity (scanning the standard solution of PPH), filter paper validation and linearity. Sustained release pellets were fabricated containing PPH by solution/suspension layering technique. A solution of PPH was prepared in distilled water and UV spectrum was taken using Perkin Elmer, Lambda25-UV/Vis Spectrophotometer. Filter paper validation was done by filtering standard PPH solution (10 ppm) through Whatman filter paper No. 41 and absorbances of filtered solution was taken repetitively and compared with unfiltered solution at 205 nm. There was negligible changed in absorbance was obtained. The data for calibration plot showed good linear graph with r2=0.9980 for PPH. According to international conference on harmonization (ICH) guidelines, the present method was validated for precision, repeatability and recovery.

Keywords

UV-Visible Spectrophotometer, Phenylpropanolamine Hydrochloride, Sustained Release Pellets.

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M. B. Mundlik ¹, C. K. Gadewar ¹, N. A. Chandekar ¹, N. M. Mahajan ¹, P. D. Telgote ¹, A. V. Chandewar ¹

Affiliations
1 Dept. of Pharmaceutical Chemistry, P. Wadhwani College of Pharmacy, Dhamangaon Road, Yavatmal-445001, IN

Abstract

A simple, selective, rapid and precise reverse phase HPLC method has been developed for the simultaneous estimation of Atorvastatin calcium and Ezetimibe in pharmaceutical dosage form. A Hypersil BDS (250 mm×4.6 mm i.d 5μ) column was used for Separation. The mobile phase was Acetonitrile:water:Methanol (350:550:100, v/v) and adjust Ph to 4.0 with Orthophosphoric acid. Flow rate 2.0 ml/min with detection at 250 nm. The retention time of Atorvastatin calcium and Ezetimibe was 21.712 and 10.414 min. respectively. The developed method was validated in terms of accuracy, precision, Linearity, specificity and forced degradation, robustness, solution stability, system suitability, limit of detection. The proposed method can be used for these drugs in combined dosage forms. The proposed RP-HPLC Method for the simultaneous estimation of Atorvastatin calcium and Ezetimibe in combined dosage form is accurate, precise, linear, rugged, robust, simple, rapid and selective. It can be easily adopted for routine quality control (QC) analysis of raw materials, formulation studies. pH of the mobile phase is 4, which is good to increase the shelf life of the column.

Keywords

RP-HPLC, Atorvastatin Calcium, Ezetimibe.

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R. A. Hajare ¹, R. M. Gaurkhede ¹, P. P. Chinchole ¹, A. V. Chandewar ¹, A. S. Wandhare ¹, S. S. Karki ²

Affiliations
1 P.W. College of Pharmacy, Dhamangaon Road, Yavatmal-445001 (M.S.), IN
2 K.L.E. College of Pharmacy, Rajaji Nagar, Bangalore-560010, IN

Abstract

Isatin (1H-indole-2, 3-Dione) is synthetically versatile molecule. Erdman and Laurent first obtained it in 1841 as a product from the oxidation of indigo by nitric acid and chromic acid. Isatin (Indole 2’3 dione), its 5-halo derivatives were reacted to form the Schiff’s bases,Mannich bases and Friedal Craft Alkylation’s to form C-C, C-N, C=N bonds. From the spectral studies it reveals that the isatin were undergoes reaction at C-3 and N-1 position. The structures of the synthesized derivatives were characterized from ¹H-NMR, IR spectral data and elemental analysis. The most of the compound synthesized were shown to possess biological activity.

Keywords

Isatin, Schiff’s Base, N-Benzylation, Dimethyl Formamide.

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